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ABclonal Biotechnology
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Image Search Results
Journal: Scientific Reports
Article Title: Activation of Wnt/β-catenin signalling via GSK3 inhibitors direct differentiation of human adipose stem cells into functional hepatocytes
doi: 10.1038/srep40716
Figure Lengend Snippet: ( a ) Protocol for the definitive endoderm induction, hepatic induction, and hepatic maturation. ( b ) Gene expression in hASCs and differentiated cells at day 5 was analysed. *Statistical significance compared to hASCs, p < 0.05. Flow cytometric analysis of FOXA2 ( c ) and CXCR4 ( d ) expression. ( e ) Immunofluorescence staining for GATA4 in hASCs and differentiated cells. Bars, 75 μm. ( f ) Expression of GATA4 in the nuclei of hASCs and differentiated cells was determined using HCS platform.
Article Snippet: The cells were then incubated with rabbit anti-β-catenin (Cell Signalling Technology, Danvers, MA,USA) at 1:100, rabbit anti-GATA4 (Merck Millipore, Germany) at 1:100,
Techniques: Expressing, Immunofluorescence, Staining